Part:BBa_J70600:Design

VioB

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2523
  Illegal AgeI site found at 2719
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Primer Design Notes:

Forward primer: gtttcttcgaattcgcggccgcttctagatgagcattctggatttcccgcgtatc l: 55 P(g/b): g Ho(max#): -114.97 Het(max#): -114.97 Tm: 70.0 GC(%): 50.9

Reverse primer: gtttcttcctgcagcggccgctactagtattattaggcctcgcggctcagtttg l: 54 P(g/b): b Ho(max#): -111.67 Het(max#): -114.97 Tm: 70.7 GC(%): 53.7

Source

designed